Data are representative of two independent experiments. ( c) Number of total cells from spleen, mesenteric lymph node (MLN) and peripheral lymph node (PLN) of age- and sex-matched Ube2n Treg-KO and Ube2n +/+Foxp3 GFP-Cre (WT) 10 week old mice presented as the mean±SD value. Results were from a total of three independent experiments (5–7 mice/genotype in each experiment). ( b) Bodyweight of Ube2n Treg-KO and Ube2n +/+Foxp3 GFP-Cre (WT) measured at the indicated times and presented as mean±SD. Songs from the Apple Music catalog cannot be burned to a CD.( a) immunoblot of Ubc13 expression in CD4 + T cells and T reg cells sorted from Ube2n fl/flFoxp3 GFP-Cre (KO) and Ube2n +/+Foxp3 GFP-Cre (WT) mice. iTunes-compatible CD or DVD recorder to create audio CDs, MP3 CDs, or backup CDs or DVDs.Internet connection to use Apple Music, the iTunes Store, and iTunes Extras.Screen resolution of 1024x768 or greater 1280x800 or greater is required to play an iTunes LP or iTunes Extras.To play 1080p HD video, a 2.4GHz Intel Core 2 Duo or faster processor, 2GB of RAM, and an Intel GMA X4500HD, ATI Radeon HD 2400, or NVIDIA GeForce 8300 GS or better is required.To play 720p HD video, an iTunes LP, or iTunes Extras, a 2.0GHz Intel Core 2 Duo or faster processor, 1GB of RAM, and an Intel GMA X3000, ATI Radeon X1300, or NVIDIA GeForce 6150 or better is required.To play standard-definition video from the iTunes Store, an Intel Pentium D or faster processor, 512MB of RAM, and a DirectX 9.0–compatible video card is required.PC with a 1GHz Intel or AMD processor with support for SSE2 and 512MB of RAM.
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